Locus: CC2716 Primer Set: 1 CC2716F (Forward) GTTGACATGATCCGAAAGAG CC2716R (Reverse) CAAACGCAAATACTGAAAGG PCR Condition Anneal. temp.: 57 PCR cycle: 40 MgCl2: 2 mM Product size: 1000 bp Restriction Enzyme: MspI PIC: 0.3 Restriction Enzyme: DdeI PIC: 0.3 Restriction Enzyme: TaqI PIC: 0.3 Restriction Enzyme: Bst0I PIC: 0.3 Restriction Enzyme: NciI PIC: 0.3