Locus: CC3367 Primer Set: 1 CC3367F (Forward) AGAGATGGCGCTCACTCATT CC3367R (Reverse) TACTACACCACCGCTTGCAG PCR Condition Anneal. temp.: 65 PCR cycle: 40 MgCl2: 2 mM Product size: 1200 bp Restriction Enzyme: NsiI PIC: 0.3